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Cytogenetics

Victorian Cancer Cytogenetics service

 

The Victorian Cancer Cytogenetics Service

Overview

The Victorian Cancer Cytogenetics service is a fully NATA/RCPA accredited laboratory which provides a state wide service for cancer cytogenetic analysis.

Cytogenetics has become an integral part of the management of haematological and other malignancies by providing important diagnostic and prognostic information. As a marker of disease status cytogenetics can be used to assess remission or relapse, including the monitoring of patients post bone marrow transplantation.

How to Contact Us

The department is located on the 2nd floor of St.Vincent's Hospital, Inpatient Services Building.
Should any questions arise regarding a specimen, test, or a result, please contact the VCCS on tel: 03 9231 4154 for consultation with the director: Assoc.Prof. Lynda Campbell, the principal scientist: Fran O’Malley or the Clinical Fellow Dr Meaghan Wall.

Cytogenetic tests performed by VCCS

  • G-Banded Chromosome analysis
  • Fluorescence In-situ Hybridisation (FISH) analysis (see attached list of clinical settings PDF [43 KB])
  • M Band and M FISH analysis (where relevant)
  • Paraffin Embedded Tissue (FFPE) FISH (after consultation)

Specimen Delivery to VCCS

All specimens are to be addressed and delivered to:

Pathology Specimen Reception,
Attn: Victorian Cancer Cytogenetics Service
2nd Floor, Inpatient Services Building
St. Vincent’s Hospital
Princes Street, Fitzroy, 3065
Tel: 03 9231 4154.

  • All specimens must be accompanied by a signed request form with cytogenetics clearly requested.
  • Specimens sent Monday to Friday should reach the department no later than 5.00p.m.
  • If taken later in the day, keep specimen at room temperature (RT) and send to department the next morning.
  • Ensure specimen is not exposed to extremes of temperature during transit.

NOTE: Do NOT place on ice!

  • Specimens taken late Friday may be sent overnight, to arrive on Saturday.
  • If taken at the weekend or on a Public Holiday, keep the specimen at RT; send first thing the next working day.
  • New leukaemia samples must be handled on the same day as obtained.  Therefore, if taken after hours, contact St. Vincent’s Hospital for the pathologist/scientist on call.

Urgent Results

If an urgent result is required, please follow-up the despatch of the specimen with a telephone call to the department and the specimen will be processed more rapidly.
An urgent result may be provided within 24-48 hours. Completed analysis with banding should take no more than 5 days.

Specimen types accepted by VCCS

Minimum Specimen Labelling Requirements

  • Label sterile tube with patient’s name (surname and first name), sex, date of birth, UR number, date specimen taken.
  • Place in biohazard bag together with completed interhospital referral form (obtainable from VCCS).

Sample Collection

Bone Marrow

  • Bone marrow (0.5 – 1.0mL) is collected into a sterile 10mL tube containing liquid Sodium Heparin.  Commercially available tubes are NOT generally suitable because of the difficulty in adequately mixing the small specimen volume with the dry heparin coating on the tube.
  • Bone marrow collection tubes are available from the VCCS.  These are sterile, 10mL tubes containing 1000 IU/mL of liquid Sodium Heparin.  They must be stored at 4oC until used.
  • Supply of these tubes can be requested by telephone and is to be picked up by courier from cytogenetics department.  All pick-up arrangements are the responsibility of the requesting institution unless prior arrangements have been made with the VCCS.

Blood Specimens

  • Peripheral blood (5-10mL) is collected into a sterile 10mL tube containing sodium heparin.  These tubes are commercially available, but if unobtainable a sterile plain 10mL tube with 1000IU/mL of liquid sodium heparin added, will suffice.
  • In the absence of Sodium heparin tubes, LITHIUM HEPARIN without gel is acceptable (although not optimal).
  • EDTA tubes are NOT acceptable.

Trephine

  • In the absence of adequate aspirated bone marrow specimen, a small piece of trephine may be sent in the same collection tube as for bone marrow or blood but with a covering of tissue culture medium for transport.
  • As trephine cultures are often unsuccessful, a preferred tissue for culture may be peripheral blood (PB) for acute leukaemias or lymphoproliferative disorders if there is a significant abnormal cell population in PB.

Lymph Node or Solid Tumors

Please see requirements for tumour specimens accepted by the VCCS PDF [51 KB] for more details.

FISH on paraffin-embedded tissue

See attached referral form PDF [55 KB] for sending FISH on paraffin-embedded tissue to VCCS.

Mission Statement

We aim to provide a comprehensive cancer cytogenetics service to the State of Victoria with a commitment to quality and efficiency of service.  We are also committed to providing education and to continuing research in the areas of cancer cytogenetics and molecular genetics.

Understanding Leukaemia

The 'Understanding Leukaemia' research project is aimed at identifying the genetic mechanisms that cause leukaemia, with targeted aims to reduce occurrence, improve treatments and increase survival rates.
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