Test Name: Sputum - Mycobacteria investigation 
Alternate Test Names AFB (Acid and Alcohol Fast Bacilli)- sputum; Atypical Mycobacteria (Mycobacterium avium complex)-sputum; Mycobacteria investigation- sputum; Respiratory Investigation for Acid-Fast Bacilli; TB investigation- sputum 
PLS Test Code TB  Laboratory Microbiology 
Specimen Sputum  Analysis Performed On  
Container Urine/Specimen container 50ml  Container colour Yellow 
Multiple specimens  
Required Volume Adult (mL)   Req Vol Paediatric (mL)  
Reference Ranges  
Turnaround Time Routine   Turnaround Time Urgent  
Turnaround Other Microscopy < 8 hours Culture <6 weeks 
Days test is performed Daily Monday to Friday  Transport 4 degrees C (On Ice) 
Special Requirements Three early morning specimens 
Patient Preparation Give the patient three yellow top 50ml containers. Make sure they are clearly labelled with patient details and labelled Specimen 1,2 and 3. Three sputum specimens must be collected each produced on a different day. Delivery on the day of collection is preferred. Specimens must be refrigerated at 4 degrees C. 
Collection Instructions If patient is ordered a nebulizer, administer prior to specimen collection. Explain to the patient that a deep cough is needed to produce sputum. The first early morning specimen is preferred by Pathology Department. Instruct the patient to rinse his/her mouth with water. If the patient has dentures they are to be removed. Instruct the patient to take a deep breath then cough, collect the specimen directly into the yellow screw top container and seal. Check there is an adequate specimen of sputum and not saliva in the container. A single specimen is adequate for M&C, cytology, fungal and TB cultures. Specimens for Cytology must be sent immediately to prevent deterioration of the exfoliated cells. Dispatch labelled specimen to Pathology Department with completed request form. 
Clinical Information  
Sent to Reference Laboratory No 
Ref Laboratory Name  
Estimated Patient Fee N/A 
Last Updated 25-Aug-2006 (Version: 1.00)