Test Name: Sputum, Microscopy and Culture 
Alternate Test Names Respiratory Specimen, Microscopy and Culture 
PLS Test Code RESP  Laboratory Microbiology 
Specimen Sputum  Analysis Performed On  
Container Urine/Specimen container 50ml  Container colour Yellow 
Multiple specimens  
Required Volume Adult (mL)   Req Vol Paediatric (mL)  
Reference Ranges  
Turnaround Time Routine   Turnaround Time Urgent  
Turnaround Other Microscopy < 8 hours Culture, bacterial < 2 days 
Days test is performed Daily  Transport 4 degrees C (On Ice) 
Special Requirements Bronchial suction trap containers are available from the laboratory 
Patient Preparation If patient is ordered a nebulizer, administer prior to specimen collection. 
Collection Instructions If TB is suspected, 3 early morning sputum specimens will be required. Explain to the patient that a deep cough is needed to produce sputum. The first early morning specimen is preferred by Pathology Department. Instruct the patient to rinse his/her mouth with water. If the patient has dentures they are to be removed. Instruct the patient to take a deep breath then cough, collect the specimen directly into the yellow screw top container and seal. Check there is an adequate specimen of sputum and NOT saliva in the container. A single specimen is adequate for M&C, cytology, fungal and TB cultures. Specimens for Cytology must be sent immediately to prevent deterioration of the exfoliated cells. Dispatch labelled specimen to Pathology Department with completed request form. 
Clinical Information  
Sent to Reference Laboratory No 
Ref Laboratory Name  
Estimated Patient Fee N/A 
Last Updated 25-Aug-2006 (Version: 1.00)